- cypherpunks - lists.cpunks.org

Chloroquine is "highly effective" at inhibiting reproduction of Novel Coronavirus nCov in cell culture.
by jim bell 20 Dec '20

20 Dec '20

https://www.dailymail.co.uk/health/article-8184997/Doctors-worldwide-say-ma… Doctors around the globe report that the malaria drug hydroxychloroquine seems the most effective treatment they've tried for coronavirus patients - but less than half as many doctors are prescribing it in the US as in other hard-hit countries like Spain. A survey of 6,200 doctors around the globe reveals that while few corners of the world are untouched by the virus, the pandemic is being handled very differently from country-to-country. And in some measures, the US continues to fall behind other nations' responses. For example, an American waits an average of four to five days to get results back after being tested for COVID-19. Half of doctors in Europe and most in China get the test results back within 24-hours. Dr Murali Doraiswamy, an adviser to Sermo, urged that countries should take note of what is working for doctors and governments elsewhere and move quickly to adopt practices that are saving lives. Hydroxychloroquine (pictured) was deemed the most effective coronavirus treatment comared to other options by more doctors worldwide than any other in a global survey =========================================================== On Sat, Feb 8, 2020 at 9:18 PM jim bell <jdb10987(a)yahoo.com> wrote: jim bell [chloroquine is an old-line drug typically used against malaria] [partial quote follows] https://www.asbmb.org/asbmb-today/science/020620/could-an-old-malaria-drug-… ASBMB Today Science Could an old malaria drug help fight the new coronavirus? Could an old malaria drug help fight the new coronavirus? By John Arnst February 06, 2020 Chloroquine might be getting new life as an antiviral treatment for the novel coronavirus that emerged in Wuhan, China in late 2019 and has infected some 25,000 people in more than 25 countries. For decades, the drug was a front-line treatment and prophylactic for malaria. In a three-page paper published Tuesday in Cell Research, scientists at the Wuhan Institute of Virology’s State Key Laboratory of Virology write that both chloroquine and the antiviral remdesivir were, individually, “highly effective” at inhibiting replication of the novel coronavirus in cell culture. Their drug screen evaluated five other drugs that were not effective. The authors could not be reached for comment. Though the paper is brief, John Lednicky, a professor at the University of Florida’s Emerging Pathogens Institute, found its results intriguing. “It’s interesting in that it really lacks a lot of details but, nevertheless, if you look at the data as presented, at least in vitro, it seems like chloroquine can be used as an early-stage drug,” he said. “It would be very good if these types of experiments were repeated by more laboratories to see whether the same results occur across the board.” Chloroquine is a synthetic form of quinine, a compound found in the bark of cinchona trees native to Peru and used for centuries to treat malaria. Chloroquine was an essential element of mass drug administration campaigns to combat malaria throughout the second half of the 20th century, and remains one of the World Health Organization’s essential medicines. However, after the malaria parasites Plasmodium falciparum and Plasmodium vivax began exhibiting resistance to the drug in the 1960s and 1980s, respectively, it was replaced by similar antimalarial compounds and combination therapies. Chloroquine is still widely used against the three other species of plasmodium and to treat autoimmune disorders and some cases of amebiasis, an intestinal infection caused by the amoeba Entamoeba histolytica. Chloroquine’s antiviral properties were explored in the mid-1990s against HIV and in the following decade against severe acute respiratory syndrome, or SARS, which is closely related to the novel coronavirus. In 2004, researchers in Belgium found that chloroquine inhibited replication of SARS in cell culture. The following year, however, another team at Utah State University and the Chinese University of Hong Kong evaluated a gamut of compounds against SARS replication in mice infected with the virus, finding that chloroquine was only effective as an anti-inflammatory agent. They recommended that it could be used in combination with compounds that prevent replication. Nevertheless, in 2009, the Belgian group found that lethal infections of human coronavirus OC43, a relative of SARS, could be averted in newborn mice by administering chloroquine through the mother’s milk. [end of partial quote] Also: https://www.nature.com/articles/s41422-020-0282-0 Remdesivir and chloroquine effectively inhibit the recently emerged novel coronavirus (2019-nCoV) in vitro Manli Wang, Ruiyuan Cao, Leike Zhang, Xinglou Yang, Jia Liu, Mingyue Xu, Zhengli Shi, Zhihong Hu, Wu Zhong & Gengfu Xiao Cell Research (2020)Cite this article 171k Accesses 1108 Altmetric Metrics details Dear Editor, In December 2019, a novel pneumonia caused by a previously unknown pathogen emerged in Wuhan, a city of 11 million people in central China. The initial cases were linked to exposures in a seafood market in Wuhan.1 As of January 27, 2020, the Chinese authorities reported 2835 confirmed cases in mainland China, including 81 deaths. Additionally, 19 confirmed cases were identified in Hong Kong, Macao and Taiwan, and 39 imported cases were identified in Thailand, Japan, South Korea, United States, Vietnam, Singapore, Nepal, France, Australia and Canada. The pathogen was soon identified as a novel coronavirus (2019-nCoV), which is closely related to sever acute respiratory syndrome CoV (SARS-CoV).2 Currently, there is no specific treatment against the new virus. Therefore, identifying effective antiviral agents to combat the disease is urgently needed. An efficient approach to drug discovery is to test whether the existing antiviral drugs are effective in treating related viral infections. The 2019-nCoV belongs to Betacoronavirus which also contains SARS-CoV and Middle East respiratory syndrome CoV (MERS-CoV). Several drugs, such as ribavirin, interferon, lopinavir-ritonavir, corticosteroids, have been used in patients with SARS or MERS, although the efficacy of some drugs remains controversial.3 In this study, we evaluated the antiviral efficiency of five FAD-approved drugs including ribavirin, penciclovir, nitazoxanide, nafamostat, chloroquine and two well-known broad-spectrum antiviral drugs remdesivir (GS-5734) and favipiravir (T-705) against a clinical isolate of 2019-nCoV in vitro. Standard assays were carried out to measure the effects of these compounds on the cytotoxicity, virus yield and infection rates of 2019-nCoVs. Firstly, the cytotoxicity of the candidate compounds in Vero E6 cells (ATCC-1586) was determined by the CCK8 assay. Then, Vero E6 cells were infected with nCoV-2019BetaCoV/Wuhan/WIV04/20192 at a multiplicity of infection (MOI) of 0.05 in the presence of varying concentrations of the test drugs. DMSO was used in the controls. Efficacies were evaluated by quantification of viral copy numbers in the cell supernatant via quantitative real-time RT-PCR (qRT-PCR) and confirmed with visualization of virus nucleoprotein (NP) expression through immunofluorescence microscopy at 48 h post infection (p.i.) (cytopathic effect was not obvious at this time point of infection). Among the seven tested drugs, high concentrations of three nucleoside analogs including ribavirin (half-maximal effective concentration (EC50) = 109.50 μM, half-cytotoxic concentration (CC50) > 400 μM, selectivity index (SI) > 3.65), penciclovir (EC50 = 95.96 μM, CC50 > 400 μM, SI > 4.17) and favipiravir (EC50 = 61.88 μM, CC50 > 400 μM, SI > 6.46) were required to reduce the viral infection (Fig. 1a and Supplementary information, Fig. S1). However, favipiravir has been shown to be 100% effective in protecting mice against Ebola virus challenge, although its EC50 value in Vero E6 cells was as high as 67 μM,4 suggesting further in vivo studies are recommended to evaluate this antiviral nucleoside. Nafamostat, a potent inhibitor of MERS-CoV, which prevents membrane fusion, was inhibitive against the 2019-nCoV infection (EC50 = 22.50 μM, CC50 > 100 μM, SI > 4.44). Nitazoxanide, a commercial antiprotozoal agent with an antiviral potential against a broad range of viruses including human and animal coronaviruses, inhibited the 2019-nCoV at a low-micromolar concentration (EC50 = 2.12 μM; CC50 > 35.53 μM; SI > 16.76). Further in vivo evaluation of this drug against 2019-nCoV infection is recommended. Notably, two compounds remdesivir (EC50 = 0.77 μM; CC50 > 100 μM; SI > 129.87) and chloroquine (EC50 = 1.13 μM; CC50 > 100 μM, SI > 88.50) potently blocked virus infection at low-micromolar concentration and showed high SI (Fig. 1a, b).

3 9

Assassination Politics needs to be edited down
by professor rat 20 Dec '20

20 Dec '20

Section nine could be tossed in its entirety. Its unoriginal and worse...boring. “Kropp on the other hand is a thinker. He proposes that a declaration of war should be a kind of popular festival with entrance-tickets and bands, like a bull fight. Then in the arena the ministers and generals of the two countries, dressed in bathing-drawers and armed with clubs, can have it out on themselves. Whoever survives the country wins. That would be much simpler and more than just this arrangement, where the wrong people do the fighting...” https://theanarchistlibrary.org/library/john-filiss-war-by-assassination

1 0

Antisemitism is practically synonymous with fascism
by professor rat 20 Dec '20

20 Dec '20

With that in mind lets turn to the exotic ' GovCorp ' conspiracy theory thats also indulged here. Karl Marx, Capital, Volume One, Part II: The Transformation of Money and Capital CHAPTER FOUR: THE GENERAL FORMULA FOR CAPITAL: "The capitalist knows that all commodities, however scurvy they may look, or however badly they may smell, are in faith and in truth money, inwardly circumcised Jews ..." GovCorp true believers here include Gramps, Darkness and Batshit. And of course the resident Marxists include Jamesd and John Young. So its a regular Nazbol Turds convention. At least till the anarchists get here.

1 0

ABC News: Hacked networks will need to be burned 'down to the ground'
by professor rat 20 Dec '20

20 Dec '20

Now Batshit Nazi Fag Moron Eunuch loser Jew, what makes you think the slack I cut Jim, translates in any way to you, Mr Jacob Appelbaum-loving PENTAGON JEW! FUCK OFF!

1 0

ABC News: Hacked networks will need to be burned 'down to the ground'
by jim bell 19 Dec '20

19 Dec '20

ABC News: Hacked networks will need to be burned 'down to the ground'. https://abcnews.go.com/Business/wireStory/hacked-networks-burned-ground-748…

2 1

Whit Diffie still needs killing ( My 200$ )
by professor rat 19 Dec '20

19 Dec '20

“Diffie is, in many ways, a member of the NSA family.” https://www.wired.com/story/plaintext-nsa-virtually-mia/

1 0

Gramps and Darkness the Dobbs and Pirro of cypherpunks
by professor rat 19 Dec '20

19 Dec '20

https://www.palmerreport.com/analysis/fox-news-forced-to-finally-crack-down… Grarpamps and Harkness ought to be on TV

1 0

Everyone a remailer
by professor rat 19 Dec '20

19 Dec '20

https://www.coindesk.com/online-privacy-pandemic-year In 2021, decentralization’s role in supporting end-to-end encryption will become more important than ever. While end-to-end encryption will remain the sine qua non of digital privacy, decentralization will play a crucial role in ensuring its resilience ..."

1 0

2027 - the death of all Religions and Nations
by professor rat 19 Dec '20

19 Dec '20

But don't just listen to me "...networks are taking a power role once reserved for god and the state https://www.coindesk.com/balaji-srinivasan-communist-woke-crypto-capital

1 0

ScienceAlert: Quantum Experiment Reveals Particles Can Form Collectives Out of Almost Nothing
by jim bell 19 Dec '20

19 Dec '20

ScienceAlert: Quantum Experiment Reveals Particles Can Form Collectives Out of Almost Nothing. https://www.sciencealert.com/quantum-simulator-reveals-that-phase-transitio… Jim Bell's comment:"Commie particles"

1 0